Part:BBa_K4201011:Design
Green Fluorescent Protein
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 228
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 641
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 228
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 228
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 737
Design Notes
Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our GFP reporter. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.
Source
This super folding Green fluorescent protein sequence is derived from Aequorea victoria1 and is a common reporter gene used in many different organisms. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.
References
1. Yang, S. et al. An efficient Agrobacterium-mediated soybean transformation method using green fluorescent protein as a selectable marker. Plant Signal. Behav. 14, 1612682 (2019).
2. Jefferson, R. A. The GUS reporter gene system. Nature 342, 837–838 (1989).
3. Donaldson, L. Autofluorescence in Plants. Molecules 25, 2393 (2020).